Affiliation

¹Department of Chemistry, Faculty of Science, Al-Baath University, Homs, Syria

²Department of Drug Control, Faculty of Pharmacy, Al-Baath University, Syria

Corresponding Author

Mohammad Anas Alfeen, Department of Chemistry, Faculty of Science, Al-Baath University, Homs, Syria; E-mail: mailto:chem_anas@yahoo.com

Citation

Alfeen, A.M., et al. Determination of Mefenamic Acid in Pharmaceutical Formulation and Wastewater by RPHPLC. (2017) J Anal Bioanal Sep Tech 2(2): 85- 88.

Copy rights

© 2017 Alfeen, A.M. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

 This study presents a chromatographic analysis method for the determination of mefenamic acid in pharmaceutical preparations and treated wastewater from the pharmaceutical industry in East Asia and specifically (Syria). An isocratic RP-HPLC method has been developed for determination of Mefenamic acid on a Grace, alltima C₁₈ column (250 x 4.6 mm, 5.0 μm) using a mobile phase consisting of (1%) Triethylamine aqueous buffer adjust pH = 2 by H₃PO₄ (85%): Methanol: Acetonitrile); (35: 20: 45 v\v\v %) at a flow rate of 2 mL/min. Detection was carried out at 220 nm. Retention time of Mefenamic acid was 7.85 ( ± 0.36) mins. The method was validated with respect to specificity, linearity, accuracy, precision, ruggedness, and robustness.

 The proposed method is simple, precise, sensitive, and reproducible and is applicable for quantification of Mefenamic acid in Tablets formulations and Wastewater developed and formulated in our laboratory.