An Overview of the Experimental Islet Isolation In Human Pancreas
Kursun, A.O1,2 Kasapoglu, P1,2, Aydin B1,2, Adas CU1,2, Ozdemir A1,2, Cevik A4, Kucuk M4, Cakiris A5, Kuçuksezer UC2, Ustek D5, Suzergoz F1,2,6,Bakkaloglu H7, Aydin AE7, Deniz G2, Karsidag K8, Yilmaz MT1,2,8
Affiliation
- 1Diabetes Application and Research Center (DIYAM), Istanbul University, Sehremini, Istanbul
- 2Department of Immunology, Institute of Experimental Medicine (DETAE), Istanbul University, Sehremini, Istanbul
- 3Department of Medical Pharmacology, Istanbul Medicine Faculty, Istanbul University, Capa, Istanbul
- 4Department of Laboratory Animals Science, DETAE, Istanbul University, Sehremini , Istanbul
- 5Department of Genetics, DETAE, Istanbul University, Sehremini , Istanbul
- 6Department of Biology, Faculty of Arts and Sciences, Harran University, Sanliurfa
- 7Department of General Surgery, Transplantation Unit, Istanbul Medicine Faculty, Istanbul University, Capa, Istanbul
- 8Department of Internal Medicine, Endocrinology and Metabolism Diseases Division, Istanbul Medicine Faculty, Istanbul University, Capa, Istanbul- Turkey
Corresponding Author
Ali Osman Gurol, Associate Professor, Diabetes Application and Research Center (DIYAM), DETAE Building, Istanbul University, 34393, Sehremini / Istanbul- Turkey. Tel: +90 544 794 3144; E-mail: ogurol@istanbul.edu.tr
Citation
Gurol, A.O., et al. An Overview of the Experimental Islet Isolation in Human Pancreas.(2015) Cell Immunol Serum Biol 1(1): 1- 3.
Copy rights
©2015 Gurol, A.O. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License
Keywords
Abstract
Type 1 diabetes mellitus (T1DM) is a disease caused by autoimmune destruction of the pancreatic beta cells. Allogeneic islet transplantation is recommended for “brittle T1DM patients “ as intensive insulin therapy seems to be associated with an increased incidence of hypoglycemia. The aim of this article was to describe the studies on human islet isolations verified in the Diabetes Application and Research Center (DIYAM) in Istanbul University. Pancreas were harvested from brain-dead donors and stored at -80°C. Media preparation [dilution, priming, wash, final wash, decontamination and gradient purification solutions, University of Wisconsin (UW) pre-purification media]; instrument set-up (dissection/perfusion and dissociation systems); distension [pancreas preparation (trimming and rinsing of the pancreas, cannulation into the pancreatic duct)]; making enzyme solution (reconstitution of enzyme, dilution of enzyme solution, filtration of diluted enzyme solution); perfusion (perfusion to distend pancreas with enzyme solution); dissociation (pancreas digestion, digested tissue collection); recombination and purification (using COBE 2991cell processor) were the main phases of islet isolations. As the primary goal of isolating the pancreatic islets for either in vivo transplantation or in vitro studies is to achieve a large amount of viable islets, also viability experiments both in vitro and in vivo using experimental animals were realized in these studies. An average of 300,000 islets were obtained after purification. We suggest that improvement in amount and viability of isolated islets after long-term storage can be achieved when appropriate storage solutions and new types of enzymes would be used, and in the near future DIYAM would be among islet transplantation centers worldwide by starting auto-transplantations of islets of Langerhans for mantaining insulin production and secretion in pancreotectomized patients.
