Affiliation

• ¹Department of Chemistry, Mekelle University College of Natural and Computational Science, Ethiopia
• ¹Department of Biology, Mekelle University, Ethiopia

Corresponding Author

Teklit Gebregiorgis Amabye, Department of Chemistry, Mekelle University College of Natural and Computational Science, Ethiopia, E-mail: teklitgeb@gmail.com

Citation

Amabye, T.G, et al. Antibacterial Activities of Nanoparticles of Titanium Dioxide, Intrinsic and Doped with Indium and Iron. (2016) J Med Chem Tox 1(1): 8-14.

Copy rights

© 2016 Amabye, T.G. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

  The need for new antimicrobial compounds has been attention on developing new and emerging materials based on nanoparticles with antimicrobial activity. The aim of this research was to evaluate the antibacterial activities of nanoparticles of titanium dioxide, intrinsic and doped with Indium and Iron in Escherichia coli and Staphylococcus aureus. The bacteriostatic effect of TiO₂ nanoparticles (two samples), TiO₂: Fe and TiO₂:In with concentrations of 50, 250 500 μg/ml. It was observed by optical density measurements. The bactericidal effect was determined by plate count agar Muller-Hinton, where they were incubated for 12 hours: 50 μl of bacterial suspension (concentration 1.5 x 10⁶ bacteria/ml), concentration 50 μl of nanoparticles suspended at concentrations between 39 and 2500 μg/ml. Then, to 10 - 6 dilutions were made and plated on agar for colony counts. There were significant decreases P < 0.05 in the bacterial optical densities with respect to control, using TiO₂ nanoparticles prepared with different contents of acetic acid at concentrations of 250 and 500 μg/ml versus E. coli and S. aureus. In the plate counts, there was a significant reduction, P < 0.05 in the number of CFU of E. coli using TiO₂ nanoparticles (50% AcAc) in concentrations 39 to 2500 μg/ml; in the case of S. aureus decrease seen in concentrations 78 to 2500 μg/ml. In both bacteria, we observed decreased bacterial growth with TiO₂ nanoparticles at concentrations of 156 - 2500 μg/ml. The two variants of preparing TiO₂ nanoparticles have higher intrinsic activity against E. coli and S. aureus, while nanoparticles doped with Indium and Iron, not power the antibacterial effect.