Affiliation

• ¹Pferdeklinik Kirchheim, Nürtinger Straße 200, 73230 Kirchheim unter Teck, Germany
• ²P.O. Box 1243, 72072 Tübingen, Germany
• ³Bahnhofstr. 20, 71560 Sulzbach, Germany
• ⁴Institute for Anatomy and Cell Biology, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany

Corresponding Author

Dr. Thomas Skutella, Professor, Institute for Anatomy and Cell Biology, University of Heidelberg, Im Neuenheimer Feld 307, INF 307, 69120 Heidelberg, Germany, Tel: +49-6221-54-8228; Fax +49-6221-54-5604; E-mail: skutella@ana.uni-heidelberg.de

Citation

Weber K., et al. Autologous Adipose Tissue-Derived Mesenchymal Stem Cells Affect the Regeneration of Equine Tendon Lesions (2016) J Vet Sci Animal Welf 1(1): 1- 10.

Copy rights

© 2016 Weber K. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

  Autologous adipose tissue-derived mesenchymal stem cells (ADSCs) are capable of mesenchymal multi-lineage differentiation including tenocyte-like cells when derived from fat tissue. Clinical data from sports horses with limb superficial digital flexor tendons (SDFT) and suspensory ligament (SL) injuries treated with ADSCs were evaluated in this retrospective study. ADSCs were recovered from subcutaneous tissue of the tail-head. In FACS analysis cultured cells were CD14⁺/CD105⁺/CD90⁺/CD44⁺ and CD34^-/CD45^-/CD117^- and stained CD90⁺/CD44⁺ in immunocytochemistry. After 15 – 30 days of post-diagnosis, we injected ADSCs intralesionally, guided by ultrasound. At regular periods of time an ultrasonography of SDFT and SL injuries and a clinical examination was conducted. In post-treatment, the ultrasonography signal was clearly improved in ADSC-treated SDFT and SL injuries compared to the lesionsite before. The injection of ADSCs did not cause any complications, nor showed any treatment-related adverse events, neither was injection flares substantiated in any of the horses. The healed lesions proved to be stable over a long period of time. For the therapy of sports horses active in different athletic disciplines, acute and chronic pain or fore- and hind-limb SDFT and SL injuries, this effect strongly supports the therapeutic use of regenerative ADSC sculptured from adipose tissue.

Abbreviations :
  ADSC: Adipose Derived Stem Cell; MSC: Mesenchymal Stromal Cell; SDFT: Superficial Digital Flexor Tendon; SL: Suspensory Ligament; ESWT: Extracorporeal Shock Wave Therapy; PRP: Platelet Rich Plasma; ACS: Autologous Conditioned Serum; AT: Adipose Tissue; SVF: Stromal Vascular Fraction