Affiliation

Laboratorio de Control de Calidad de Medicamentos, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral

Corresponding Author

De Zan, M.M. Laboratory of Quality Control of Medicines, Faculty of Biochemistry and Biological Sciences, National University of the Littoral, Santa Fe, Argentina; Tel: + 54 342 4575205; E-mail: mmdezan@fbcb.unl.edu.ar

Citation

De Zan, M.M., et al. Bioanalytical Method Optimization for the Therapeutic Drug Monitoring of Vancomycin. (2017) J Anal Bioanal Sep Tech 2(2): 89- 95.

Copy rights

© 2017 De Zan, M.M. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

         Chemometric optimization and validation of a method based on High Performance Liquid Chromatography (HPLC) using core – shell particles for the determination of Vancomycin (VMC) in human plasma is reported. The combination of the efficiency of the core-shell particles and the benefits of the design of experiments allowed the successful determination of VCM, even in presence of several interferents. Selectivity, linearity, accuracy and precision were accomplished according to the European Medicines Agency (EMA) guideline, within the concentration range of 1.00 – 60.0 μg/mL of VCM. It is noteworthy that this method requires small amount of sample and solvents, and the sample treatment is simple and no time-consuming. Thus, this method becomes a simple and high-throughput alternative to therapeutic drug monitoring in treated patients, as well as an analytical procedure that conforms to the principles of the green chemistry.