Affiliation

¹Department of Chemistry and Biomolecular Sciences, Faculty of Science, Macquarie University, NSW 2109, Australia

²Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, NSW 2109, Australia

³Wollongong Hospital, South Eastern Sydney and Illawarra Area Health Service, Wollongong, NSW 2500, Australia

Corresponding Author

Mark S. Baker, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Macquarie University, NSW 2109, Australia, Telephone: +61298508211; E-mail: mark.baker@mq.edu.au

Citation

Abidali Mohamedali., et al. Cross Species applicability of Anti-human plasma IgY affinity column. (2016) Cell Immunol Serum Biol 2(1): 34- 38.

Copy rights

© 2016 Mark S.B. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

  In order to improve the sensitivity of proteomic analysis, our group has developed a chicken antibody based column that has successfully depleted high abundance proteins (HAPs) from human plasma prior to mass spectrometry analysis. The use of chicken as a host for polyclonal antibodies production conveys many advantages, including but not limited to, the capabilities of producing large quantities of IgY antibodies with minimal antigen quantities and enhanced immunogenicity exhibited by their immune systems against foreign immunogens. In this study, we explored the ability to use an anti-human IgY affinity column with a variety of plasma samples derived from different animals and demonstrate this column’s ability to deplete the two most common HAPs (albumin and immunoglobulins) reproducibly in phylogenetically related species. Hence, our study highly recommends that IgY-based immune depletion technologies could be used to capture targeted proteins among phylogenetically related species without having to produce separate columns for each clade.