Detection and Quantification of Subgingival Plaque Bacteria in Beagle Dogs
Serafim A. Papadimitriou1*, Maria I. Kouki1, Ioanna Papadopoulou2, Theodora Slini3, Dimitra Sakellari4
Affiliation
- 1Companion Animal Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
- 2Private Practitioner, Thermi, Thessaloniki, Greece
- 3Laboratory teaching staff, Laboratory of Heat Transfer and Environmental Engineering, Department of Mechanical Engineering, Aristotle University of Thessaloniki, Greece
- 4Department of Preventive Dentistry, Periodontology and Implant Biology, School of Dentistry, Aristotle University of Thessaloniki, Greece
Corresponding Author
Serafim A. Papadimitriou, Associate professor, Companion Animal Clinic, School of Veterinary Medicine, Aristotle University of Thessaloniki 11, Voutyra str., 54627, Thessaloniki, Greece, Tel: +00302310994575; Fax: +00302310994449; E-mail: serpap@vet.auth.gr
Citation
Papadimitriou, S.A., et al. Detection and Quantification of Subgingival Plaque Bacteria in Beagle Dogs. (2016) J Vet Sci Animal Welf 1(1): 1- 5.
Copy rights
© 2016 Papadimitriou, S.A. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
Subgingival plaque is the most important aetiological factor of periodontal disease. Specific ecologic relation between subgingival microbial species of different virulence initiates gingival inflammation and subsequently causes periodontal destruction. Purpose of this study was to investigate this relationship and correlate it with the periodontal pocket depth. For this purpose, 18 whole genomic DNA probes constructed for human periopathogens and the “checkerboard” DNA-DNA hybridization were used to evaluate 128 plaque samples collected from 16 Beagle dogs. All investigated species were detected in all sites and complex microbial communities were recognized. Microbial counts and biofilm complexity seem to differ depending on the animal’s periodontal pockets depth, although these differences did not reach statistical significance. Results of the current study suggested that i) the “checkerboard” DNA-DNA hybridization technique, despite its limitations, can provide interesting information about oral microbiota in dogs ii) potentially zoonotic bacteria were detected in all dogs iii) results are in accordance with other researchers’ findings regarding the synergistic activity of oral microorganisms in dogs’ periodontal disease.
