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Publish Date : 2015-04-02
Journal of Diabetes and Obesity

Effect of Chromium(III) -Amino acid (1:3)Complexes on High Sucrose Induced Insulin Resistance, Lipid Abnormalities and Oxidative Stress in Male Sprague Dawley Rats

Raghunath Manchala
Nagabhushan Reddy Konapalli1, Anil. Sakamuri1, Kalashikam Rajender Rao1, Rajendher Reddy M2, Praveen Kumar Y2, Sirasani Satyanarayana2 and Raghunath Manchala1*
Article information 

Affiliation

  • 1National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, 500 007, India.
  • 2Department of Chemistry, Osmania University, Hyderabad, 500 007, India.

Corresponding Author

Dr. Raghunath Manchala, Scientist-F & Head, Division of Endocrinology & Metabolism, National Institute of Nutrition, Jamai Osmania, Hyderabad -500 007, Andhra Pradesh, India. Tel:91-40-27197235/ FAX: 91-40-27019074; E-mail: mraghunath55@yahoo.com

Citation

Manchala, R., et al. Effect of Chromium (III) -Amino Acid (1:3) Complexes on High Sucrose Induced Insulin Resistance, Lipid Abnormalities and Oxidative Stress in Male Sprague Dawley Rats. (2015) J Dia Obes 2 (1): 16- 23.

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© 2015 Manchala, R. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

 

  This study was carried out to assess the anti-hyperglycaemic effects if any of novel synthetic binary chromium (III)-amino acid complexes in a high sucrose (HS) induced insulin resistance (IR) and or impaired glucose tolerant male Sprague Dawley (SD) rat model. Aqueous solutions of chromium chloride hexahydrate (CrCl3.6H2O) and D/L amino acids were mixed in 1:3 molar ratios, refluxed at 80°C for 4 hours and the resultant crude greenish-violet solid was extracted with acetone, dried in a hot air oven and their elemental composition and structure were determined. The chromium- amino acid [Cr-(AA)3] complexes were administered orally (~45 μg/kg body weight/day up to 12 weeks) to adult male SD rats in which IR and or impaired glucose tolerance were induced by feeding a HS diet. In line with literature reports, Cr-D-(Phe)3 complex improved insulin sensitivity and oral glucose tolerance in the HS fed rats. Interestingly, Cr-L-(Phe)3 complex also improved these parameters. Increased phosphorylation of insulin receptor substrate- 1 and Akt and increased glucose transporter-4 expression in skeletal muscle were associated with these changes suggesting modulation of insulin sensitivity by Cr-L/D-(Phe)3 complexes. Further, sub chronic administration of Cr-(AA)3 complexes significantly improved lipid metabolism. Modulation of oxidative stress and/ or antioxidant status of the animals seem to be associated with/underlie these beneficial effects in HS induced insulin resistant SD rats.