Efficacy of Symbiotics in an Experimental Model of Endotoxic Shock Induced by Lipopolysaccharide from Escherichia Coli
Erika D. Gonzalez-Renovato1, Genaro Gabriel Ortiz1*, Fermin P. Pacheco-Moises2, Jose A. Cruz-Serrano3, Alfredo de J. Celis de la Rosa4, Oscar K. Bitzer-Quintero5, Angelica L. Sanchez Lopez1 and Moises A. Alatorre-Jimenez1
Affiliation
- 1Laboratory of Development, Aging and Neurodegenerative Diseases, Neurosciences Division, Biomedical Research Centre West (CIBO), Mexican Social Security Institute, Sierra Mojada 800, Col. Independence, 44340 Guadalajara, Jalisco, Mexico
- 2Department of Chemistry, University Center of Exact Sciences and Engineering, University of Guadalajara, Boulevard Gral, Marcelino Garcia Barragan 1421, Olympic, 44430 Guadalajara, Jalisco, Mexico
- 3Research Coordinator and Director General Company Kurago Biotech SA de CV, Moctezuma # 144 Zapopan, Jalisco, Mexico
- 4Department of Public Health. University Center for Health Sciences, East Independence, 44340 Guadalajara, Jalisco, Mexico
- 5Immunomodulation Laboratory Division of Neuroscience, Biomedical Research Centre West (CIBO), Mexican Social Security Institute, Sierra Mojada 800, Col. Independence; 44340 Guadalajara, Jalisco, Mexico
Corresponding Author
Dr. Genaro Gabriel Ortíz, Development- Aging Laboratory, Neurodegenerative diseases, Division of Neurosciences, CIBO-IMSS, Sierra Mojada 800 CP 44340. Guadalajara, Jalisco, México, Tel: (0133) 2015 3651; E-mail: genarogabriel@yahoo.com
Citation
Gonzalez-Renovato, E.D., et al. Efficacy of Symbiotics in an Experimental Model of Endotoxic Shock Induced by Lipopolysaccharide from Escherichia Coli. (2016) Int J Food Nutr Sci 3(1): 252-258.
Copy rights
© 2016 Gonzalez-Renovato, E.D. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
Obsevation: Endotoxic shock is a life-threatening condition and its sequelae represent an array of clinical symptoms that encompass systemic inflammation, coagulopathy, and abnormalities of the renal, hepatic, pulmonary and hematologic systems, followed by multi-organ failure. Furthermore, systemic failure leads to an unfavorable environment in the gut that causes an imbalance in the homeostasis of intestinal microbiota. In the present study, the effect of symbiotics as adjuvant therapy in endotoxic shock was investigated. Adult male Wistar rats were randomized into three groups: Control group received a single intraperitoneal injection of physiological saline solution, and the other two groups received either a symbiotic formulation or a placebo daily, for one week, followed by the administration of a single lethal dose of lipopolysaccharide (LPS) from E. coli (20 mg/Kg, IP).
Results: The symbiotic formulation used improved the survival rate of LPS-treated rats, ameliorated the clinical symptomatology, reduced the production of serum proinflammatory cytokines (TNF-α, IL-6, IL-1β), and preserved the mitochondrial membrane fluidity and ATPase activity.
Conclusion: The symbiotic formulation used as a pre-treatment in this experimental model, reduced mortality and showed beneficial effects at the systemic and sub-cellular levels.
