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Publish Date : 2015-09-18
Journal of Dentistry and Oral Care

In Vitro Cytotoxicity Evaluation of Polymerized Resin Cements on the Viability of L-929 Fibroblast Cells

Horieh Moosavi
Article information 

Affiliation

  • 1Dental Materials Research Center and Department of Operative Dentistry, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
  • 2Department Immunologist, Buali Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Corresponding Author

Horieh Moosavi. Dental Materials Research Center and Department of Operative Dentistry, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran. E-mail: dentist_57@yahoo.com , Moosavih@mums.ac.ir

Citation

Moosavi, H., et al. In Vitro Cytotoxicity Evaluation of Polymerized Resin Cements on the Viability of L-929 Fibroblast Cells. (2015) J Dent & Oral Care 1(4): 1-5.

Copy rights

©2015 Moosavi, H. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

 

Objective: This study was designed to evaluate the cytotoxicity of two resin-based cements; Bistite II DC, and Multi Bond II, after polymerization on cultured L-929 fibroblast cells.
Methods: Disc-shaped samples were prepared in polyethylene molds with cylindrical cavities (5 mm diameter, 2 mm high) of BistiteII DC and Multi Bond II. After setting of resin cement disc, they were aged for 1, 3, and 5 days in Dulbecco’s Modified Eagle’s Medium (DMEM). Cell viability of L-929 fibroblast cells was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the difference between the groups was tested by analysis of variance and Tukey tests (a = 0.05).
Result: After 1 day of storage, the Bistite II DC, and Multi Bond II were essentially non-cytotoxic. On days 5 of the experiment, the cell viability of two resin cements did not differ significantly (P > 0.05), but cell viability was slightly reduced on day 5. There was no significant difference in the cytotoxicity between Bistite II DC, and Multi Bond II.
Conclusion: After polymerization, two resin-based cements (Bistite II, and Multi Bond) induced slight cytotoxicity. The sensitivity of cytotoxicity to L-929 cells in depended on the type of resin-based cements.