Affiliation

• ¹Faculty of Higher Cuautitlán Izcalli, National Autonomous University of Mexico, Mexico state, MéxicO
• ²Department of Pharmacy, National School of Biological Sciences of the National Polytechnic Institute. Mexico
• ³Faculty of Chemistry , University of the State of Mexico . Mexico
• ⁴Biochemical Engineering Laboratory , National School of Biological Sciences, National Polytechnic Institute. Mexico
• ⁵Faculty of Health Sciences. Universidad Anahuac Mexico Norte. Mexico
• ^§Gutiérrez-Salmeán and Chamorro-Cevallos co-senior this manuscript

Corresponding Author

German Chamorro-Cevallos, Department of Pharmacy, National School of Biological Sciences, National Polytechnic Institute , Avenida Wilfrido Massieu s / n. Unit Adolfo Lopez Mateos, Del. Gustavo A. Madero, 07738 Mexico, DF, Mexico, Tel: + (52) (55) 5729 6000/ ext. 52398; E-mail: gchamcev@yahoo.com.mx

Citation

Chamorro-Cevallos, G., et al. Methods for Extraction, Isolation and Purification of C-phycocyanin: 50 years of Research in Review. (2016) Int J Food Nutr Sci 3(1): 275-284.

Copy rights

© 2016 Chamorro-Cevallos, G. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

Abstract

Context: Spirulina (Arthrospira) exerts a wide spectrum of pharmacological activities that are largely attributed to its phycobiliprotein content, mainly to C-phycocyanin. The extraction, isolation and purification of C-phycocyanin have been studied for many years, resulting in diverse methodologies with a range of yields and grades of purity.
Objective: We performed a systematic review of the literature, consulting all the available years in TOXNET, PubMed/MEDLINE and Science Direct-Scopus. Search criteria included the separation, isolation, and purification methods for C-phycocyanin from different microorganisms. Search words were: extraction, separation, isolation and purification of C-phycocyanin.
Results: The combination of aqueous two-phase systems for extraction and ultrafiltration for purification results in the best yields and highest purity of the desired nutraceuticals. It is also essential to consider the freshness and species of the primary biomass, as these factors heavily influence the concentration and viability of the phycobiliproteins and therefore affect the yield and purity.
Conclusion: In order to preserve the valuable properties and health benefits of nutraceuticals, such as C-phycocyanin, it is essential to seek innovative methods for isolating and purifying these bioactive substances from natural sources. The information herein gathered indicates the best methods currently available.