Molecular Identification of Staphylococcus aureus Isolated from Clinical Samples by Specific PCR Assay Targeting the Sgnal Transduction Gene
Zhiwei Zhou , Yuhao Huang , Rong Chen
Affiliation
- 1School of Life Sciences, Shanghai University, Shanghai, China
- 2Shanghai Center for Clinical Laboratory, Shanghai, China
Corresponding Author
Zhamin Liu, School of Life Sciences, Shanghai University, Shanghai, China, Tel: +86-21-66135166/ Fax: +86-21-66135166; E-mail: zhmliu@shu.edu.cn
Citation
Liu, Z., et al. Molecular Identification of Staphylococcus Aureus Isolated from Clinical Samples by Specific PCR Assay Targeting the Signal Transduction Gene Research. (2017) J Pharm Pharmaceutics 4(2): 166- 171.
Copy rights
© 2017 Liu, Z. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
Staphylococcus aureus (S. aureus) is a common, Gram-positive species that is pathogenic in both human and animals. Rapid and direct identification of this bacterium specifically from clinical specimens would be useful in improving the diagnosis of S. aureus infections in the clinical microbiology laboratory. Although, some molecular identification assays are reported, some false-positive cases are described; it is an urgent need to develop a specific diagnostic method. Currently, the sensitivity and specificity of polymerase chain reaction targeting a signal transduction gene (vick) for the diagnosis of S. aureus isolates in clinical samples was developed. The assay is sensitive enough to detect 5500 copies of a cloned fragment of the S. aureus vick gene. This PCR assay can be used for rapid clinical diagnosis of S. aureus infection and detection of the pathogen in food samples.
