New Lipopolysaccharide Binding Proteins from the Jellyfises Aurelia Aurita and Rhopilema Asamushi of Sea of Japan
Affiliation
- 1G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia
- 2Far Eastern Federal University, School of Biomedicine, Vladivostok, Russia
Corresponding Author
G.A. Naberezhnykh, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Pr. 100-letiya Vladivostoka, 159, Vladivostok, Russia, 690022, Tel: +7-4232-311406/ Fax: +7-4232-314050; E-mail: naber1953@mail.ru
Citation
Naberezhnykh, G.A., et al. New Lipopolysaccharide Binding Proteins from the Jellyfishes Aurelia Aurita and Rhopilema Asamushi of Sea of Japan. (2017) J Marine Biol Aquacult 3(2): 1- 5.
Copy rights
© 2017 Naberezhnykh, G.A. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
The commercial species of jellyfishes of Sea of Japan were studied as the sources of proteins that are capable lipopolysaccharides (LPSs) of Gram-negative bacteria. LPS-binding proteins have been found in lysates of jellyfishes mesogloea by means of immunofluorescence assay using LPS labeled with fluorescein (F-LPS). It was shown that lysate from rhopilema demonstrate the major LPS-binding activity in comparison with extracts from aurelia. For isolation and purifying of LPS-binding proteins from lysate of jellyfishes, the cation-exchange chromatography was used. It was shown, that fractions of jellyfishes lysate after cation-exchange chromatography contained several LBP with high-molecular weights. By ligand enzyme solid phase assay it was shown that isolated LBP of jellyfishes was bound to LPS directly by concentration-dependent manner in saturation process.
