Potent Anti-Adipogenic Effect of Green Tea and Green Tea Extracts in Chicken
Alemu Regassa1#, Dong-hun Lee2#, Soo-Won Choi2, Chang-Seon Song2, Woo Kyun Kim3, Jong Hwan Kwak4
Affiliation
- 1Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada
- 2College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea
- 3Department of Poultry Science, University of Georgia, 303 Poultry Science Building, Athens 30602, GA, U.S.A
- 4College of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea
- #These authors contributed equally
Corresponding Author
Woo Kyun Kim, Department of Poultry Science, University of Georgia, 303 Poultry Science Building, Athens 30602, GA, U.S.A, E-mail: wkkim@uga.edu
Citation
Kyun Kim, K., et al. Potent Anti-Adipogenic Effect of Green Tea and Green Tea Extracts in Chicken. (2017) J diab Obes 4(3): 1- 6.
Copy rights
© 2017 Kyun Kim, K. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
This study was conducted to examine the effect of green tea on body, adipose tissue, and liver weights and adipose tissue weight to body weight ratio and the adipogenic differentiation and expression of adipogenic transcripts in chicken (Gallus gallus) preadipocytes. In experiment one, chicks were weighed and randomly assigned to a control diet (CTRL) and green tea treatment (GT 1%, w/w) for 38 days. In experiment 2, preadipocytes were isolated from 20 wk old chicken and treated with an adipogenic cocktail (DMIOA) containing 500 nM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine, 20 μg/mL insulin, and 300 μM OA, DMIOA+30 μg of extract B, E, H, Mc, T, and W, respectively, for 48 h. Data were analysed using the General Liner Model procedure of the Statistics Analysis System (SAS) Institute version 9.4, and differences were considered significant at P < 0.05. Gene expression was measured using quantitative real-time PCR. GT 1% significantly reduced body (- 9%; P = 0.0447), liver (-20%; P = 0.0206), and abdominal fat weight (- 44%; P = 0.0055) compared with the control (CTRL) group (GT 0%). The abdominal fat/body weight ratio of green tea supplemented group (- 36%; P = 0.0125) was also significantly lower than that of control group. In cell culture study, all green tea extracts inhibited C/EBPα and β mRNA expression compared to DMIOA. DMIOA+B, T, or W reduced mRNA expression of FABP4 by three-fold compared to DMIOA. Although all green tea extracts reduced adipocyte formation, T and W had the strongest anti-adipogenic effects. These results demonstrate that supplementation of green tea could be an effective strategy in the control of obesity in chickens.
