Statistical Optimization of Xanthan Gum Production and its Characterization Using Pseudomonas aeruginosa NCIM (2948) in Submerged Fermentation (SMF) Process
Suheela Bhat1, Abhishak Dutt Tripathi2*, Khan Nadiya Jan1, Kamlesh Kumar Maurya2*
Affiliation
- 1Faculty of Engineering and Interdisciplinary Sciences, Jamia Hamdard New Delhi-110062, India
- 2Centre of Food Science and Technology, Banaras Hindu University, Varanasi 221005, India
Corresponding Author
Abhishak Dutt Tripathi, Centre of Food Science and Technology, Banaras Hindu University, Varanasi 221005, India, Tel: 91-9450533651; E-mail: abhi_itbhu80@rediffmail.com
Citation
Abhishak Dutt Tripathi. Statistical Optimization of Xanthan Gum Production and its Characterization Using Pseudomonas aeruginosa NCIM (2948) in Submerged Fermentation (SMF) Process. (2018) J Nanotechnol Material Sci 5(1): 8- 13.
Copy rights
© 2018 Abhishak Dutt Tripathi. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
In the present study Pseudomonas aeruginosa NCIM 2948 was used to produce exo polysaccharide xanthan using Xanthomona scampestris NCIM 5028 as control strain and efforts have been made to optimize the variables viz carbon source, nitrogen source, carbon concentration, nitrogen concentration and agitation speed for enhanced xanthan gum production. Optimization of process variables was done by central composite rotatable design (CCRD) using design expert (DX 8.0.7) software. Shake flask cultivation performed under optimum condition viz; 55 g/L carbon concentration, 5 g/L nitrogen concentration and 200 rpm agitation speed gave xanthan yield of 17 g/L using sucrose as carbon source and soya bean casein hydrolysates as nitrogen source. Batch cultivation further performed in 7.5 L lab scale bioreactor (working volume: 3L) under optimized condition gave maximum cell biomass of 13 ± 0.5 g/L with a xanthan content of 17 g/L after 72h of fermentation. Scale up study on bioreactor gave maximum xanthan yield. Xanthan yield (Yp/x) in terms of cell biomass produced was found to be 1.33 (g/g). Maximum productivity was found to be 0.24 g/Lhr which is higher than previous reports under similar condition. Characterization of xanthan gum was done by FTIR.
