Suppressive Effects of Welsh Onion Extracts on Mucus Hyper-production in Human Airway Cells
KimihikoSano, Jun Iwashita, Keishi Hata
Affiliation
- 1Sano Inc., 3-4-2 Oroshi-machi, Akita, 010-0061, Japan
- 2Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo Nakano, Akita 010-0195, Japan
- 3Akita Research Institute of Food & Brewing, 4-26 Sanuki, Arayamachi, Akita 010-1623, Japan
Corresponding Author
Akiko Takashima, Sano Inc., 3-4-2 Oroshi-machi, Akita, 010-0061, Japan, Tel: +81-18-862-6644; Fax: +81-18-862-6646; E-mail: a-takashima@sano-co.com
Citation
Takashima, A., et al. Suppressive Effects of Welsh Onion Extracts on Mucus Hyper-Production in Human Airway Cells. (2016) Lett Health Biol Sci 1(2): 34- 36
Copy rights
© 2016 Takashima, A. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.
Keywords
Abstract
We investigated the suppressive effects of Welsh onion (Allium fistulosum L.) on the hyper-production of mucins in NCI-H292 human lung cancer cells. Periodic acid-Schiff staining showed that the 50% (v/v) ethanol extract of Welsh onion suppressed mucus glycoprotein production in cells, whereas the water and ethanol extracts did not. A real-time RTPCR analysis demonstrated that the 50% ethanol extract attenuated the gene expression of MUC2 and MUC5AC, which are major airway mucus components, in NCI-H292 cells stimulated with lipopolysaccharide (LPS). Furthermore, dot blot hybridization revealed that the extract at 100 μg/ml attenuated the hyper-production of the MUC5AC protein in LPS-stimulated NCI-H292 cells.
