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Short Communication
Publish Date : 2016-05-12
Journal of Marine Biology and Aquaculture

Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides

Laurent VANDANJON
Laurent Vandanjon2,4*, Laurent Vallet1,2, Titouan Le Glatin1, Paul Deleris1, Regis Baron3, Patrick Bourseau2,4, Justine Dumay1
Article information 

Affiliation

  • 1Université de Nantes, MMS, rue de la Houssinière, Nantes, France
  • 2Université de Nantes, GEPEA, Boulevard de l’Université, Saint-Nazaire, France
  • 3IFREMER, Laboratoire BiorafHE, Unité Biotechnologie et Ressources Marines, Rue de l’Ile d’Yeu, Nantes, France
  • 4Université de Bretagne Sud, LBCM, Rue Saint-Maudé, Lorient, France
  • 5Université de Bretagne Sud, IRDL, Rue Saint-Maudé, Lorient, France

Corresponding Author

Laurent Vandanjon, Université de Nantes, GEPEA, Boulevard de l’Université, Saint-Nazaire, France: 44600, Université de Bretagne Sud, LBCM, Rue Saint-Maudé, Lorient, France: 56100, Tel: 33.2.97.01.71.25; E-mail: laurent.vandanjon@univ-ubs.fr

Citation

Vandanjon, L., et al. Valorization of the Macroalgae Sargassum muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides. (2016) J Marine Biol Aquacult 2(1): 1- 7.

Copy rights

© 2016 Vandanjon, L. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License

Keywords

Abstract

  The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates.
  After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial protease and an alginate lyase extracted from Pseudomonas alginovora led after 3 hours of hydrolysis to the solubilization of 2.43% DW polysaccharides and 0.52% DW phlorotannins. The use of 0.5% volume of the surfactant Triton® X-100 with 10% DW of the enzymatic mix has allowed to reaching the value of 2.63% DW of solubilized phlorotannins, that is 96% of the total phenolic content.
  The use of non-ionic surfactant, combined to enzymatic hydrolysis, showed an increased efficiency in disrupting cell wall and solubilizing phlorotannins in Sargassum muticum.