Affiliation

¹Department of Chemistry, Central Laboratory, Ministry of Higher Education & Scientific Research, P. O. Box Office 7099, Khartoum, Sudan

²Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

³King Faisal University, Chemistry Department, Al-Ahsaa, Kingdom of Saudi Arabia, P.B. Box 380, Hufof, 31982

Corresponding Author

Abdalla Ahmed Elbashir Ahmed, Professor of Analytical Chemistry, Chemistry Department, King Faisal University, Al-Ahsaa, Kingdom of Saudi Arabia, P.B. Box 380, Hufof, 31982, Tel: +966567254917; E-mail: aaeahmed@kfu.edu.sa

Citation

Elbashir, A., et al. Antioxidant, Antimicrobial Activities and HPLC Quantitative Analysis of Some Sudanese Medicinal Plants. (2021) J Food Nutr Sci 8(1): 1-8.

Copy rights

© 2020 Elbashir, A. This is an Open access article distributed under the terms of Creative Commons Attribution 4.0 International License.

Keywords

HPLC-PDA; Antioxidant Activity; Antimicrobial Activity; Sudanese medicinal plants; Bioactive compounds

Abstract

The objective of this study was to evaluate the antimicrobial and antioxidant activities and to determine some of bioactive components of ethanolic extract of Adansonia digitata fruit pulp, Hibiscus sabdariffa flower, Hyphaene the baica fruit pulp and Ziziphus spina Christi fruit pulp. The ethanolic extracts of the plants were tested against four bacterial strains (Escherichia coli, Pseudomonasaeruginosa, Bacillus subtilis and Staphylococcus aureus) and two fungal species (Candida albicans and Aspergillus niger) using disc diffusion method. Different plants extracts showed variable activity against all the tested microorganisms. The plant extracts were evaluated for their antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assays. The highest result of antioxidant activity by DPPH and ABTS assays was observed in Adansonia digitata fruit pulp extract (77.4%) with IC50 of 0.273 μg mL-1 and Hyphaene the baica fruit pulp extract (79.02%) with IC50 value of 0.0808 μg mL-1. The identification and quantification of ascorbic acid, gallic acid and quercetin in the samples ethanolic extracts was performed by a HPLC-PDA method. These compounds have been detected in all the tested extracts.